DNA Metabarcoding services
DNA metabarcoding is the genetic analysis of bulk samples including a mix of different organisms. This includes samples from insect traps, plankton catches, stomach contents, faeces etc.
Specimens are not handled individually but the sample is homogenized and analyzed using high throughput parallel sequencing. Sequences are then computer-processed to obtain the species composition of the mixed sample.
We process
- Zoo- and phytoplankton
- Faeces
- Gut contents
- Samples from malaise and pitfall traps
- Processed food (e.g.: cans)
Our standard analysis includes
- Sample preparation (homogenization, subsampling)
- DNA extraction (commercial kits)
- Genomic DNA quantification
- Primer selection and in-silico test
- PCR amplification
- Quality control
- NGS library preparation
- High-throughput sequencing
- Bioinformatic analysis
Sampling
The quality of DNA in the sample influences the results of our analysis. For this reason samples are ideally collected by trained personnel, fixed in an appropriate medium (Ethanol, RNA later, DMSO, DESS etc.) or frozen.
We suggest you to get in touch with us to receive support in planning your sampling, fixing and storing your samples. We gladly issue an individual offer.